师资队伍

曹博

2019年06月04日 09:45  点击:[]

曹博简介

姓名:曹博

博士,教授,博士生导师

联系方式:caobo@qfnu.edu.cn


 

教育背景和研究工作简历(从大学开始)

2004~2008 广西大学 生物技术 学士

2008~2014 上海交通大学 生物学 博士

2012~2014 美国麻省理工学院 联合培养博士生

2015~2018 美国麻省理工学院 博士后

2018~2019 新加坡SMART研究所Research   Scientist

2019~ BETVLCTOR伟德入口 bevictor伟德 教授

研究方向、承担课题及发表论文概述

本课题组研究方向为分子生物学,主要研究领域为利用基因组学、分子生物学、分析化学、生物信息学等手段,围绕DNARNA等生物大分子上的表观遗传修饰的发现、检测、功能鉴定、生物合成途径,以及DNA损伤/修复的分子机理等方向进行研究。

承担课题:

1.        国家自然科学基金面上项目,32070629,tRNA片段及RNA磷硫酰化修饰在细胞应激反应中的动态变化规律研究,2021.01-2024.12,在研,主持

2.        美国自然科学基金(NSF),1709364, Chemical and biochemical   determinants of phosphorothioate stability and location in bacterial genomes, 2017.8.1-2020.7.31,350万元,已结题,参加

3.        国家自然科学基金青年项目,31400029,沙门氏菌DNA磷硫酰化修饰蛋白复合物的功能研究,2015.01-2017.12,24万元,已结题,参加

4.        国家自然科学基金面上项目,31470183,细菌基因组上DNA磷硫酰化修饰的丰度和序列特异性,2015.01-2018.12,88万元,已结题,参加

5.        美国国立健康研究院(NIH)重大研究计划,NIHRO1-ES024615,Translational Control of   ROS Management,2014.08-2019.05,598万元,已结题,参加

6.        美国国家自然科学基金,MCB-1412379,Role of Nucleoside Modifications in tRNA Surveillance in Prokaryotes,2014.08-2017.07,100万元,已结题,参加

7.        国家重点基础研究发展计划(973计划)2012CB721004,DNA硫修饰依赖限制系统的功能分析,2012.01-2013.12,60万元,已结题,参加

科研论文:

1. Hu J, Yim D, Ma   D, Huber MS, Davis N, Bacusmo JM, Vermeulen S, Zhou J, Begley TJ, DeMott MS,   Levine SS, Crécy-Lagard V, Dedon PC*, Cao B*. Sequencing-based   quantitative mapping of the cellular small RNA landscape. 2020, BioRxive doi:  https://doi.org/10.1101/841130   (under review at Nature Biotechnology)

2. Cao B,   Yuan Y, Byrne S, Haruehanroengra P, Wu J, DeMott MS, Dedon PC*. Chemistry at   the genomic level: Sequencing tools to define the chemical landscape of   epigenetics, epitranscriptomics, and DNA damage. 2020, (invited review   article by Accounts of Chemical Research).

3. Wu X#, Cao B#, Aquino P, Chiu TP, Chen C, Jiang S, Deng Z, Chen S, Rohs   R, Wang L, Galagan JE, Dedon PC. Epigenetic competition reveals   density-dependent regulation and target site plasticity of phosphorothioate   epigenetics in bacteria. Proc NatlAcad Sci USA. 2020Jun 23;117(25):14322-14330. doi: 10.1073/pnas.2002933117.

4. Cao B*#, Wu   X#, Zhou J, Wu H, Liu L, Zhang Q, DeMott MS, Gu C, Wang L, You D, Dedon PC*.   Nick-seq for single-nucleotide resolution genomic maps of DNA modifications   and damage. Nucleic Acid Research. 2020 Jul 9;48(12):6715-6725.   doi: 10.1093/nar/gkaa473.

5. Lutz T, Czapinska H, Fomenkov A, Potapov   V, Heiter DF, Cao B, Dedon   P, Bochtler M, Xu SY*. Protein Domain Guided   Screen for Sequence Specific and Phosphorothioate-Dependent Restriction   Endonucleases. Front Microbiol. 2020 Aug 18;11:1960. doi:   10.3389/fmicb.

6. Sun Y, Kong L, Wu G, Cao   B, Pang X, Deng Z, Dedon   PC, Zhang C, You D*. DNA Phosphorothioate   Modifications Are Widely Distributed in the Human Microbiome.Biomolecules.   2020 Aug 12;10(8):1175.

7. Zhu S, Zheng T, Kong L, Li J, Cao   B, DeMott MS, Sun Y, Chen Y, Deng Z, Dedon   PC, You D*. Development of   Methods Derived from Iodine-Induced Specific Cleavage for Identification and   Quantitation of DNA Phosphorothioate Modifications. Biomolecules 2020, 10(11), 1491.

8. Kellner SM, DeMott MS, Cheng C, Russell B, Cao B,   Dedon PC*. Oxidation of labile phosphorothioate DNA modifications leads to   lethal genomic instability. Nat Chem Biol. 2017   Aug;13(8):888-894.

9. Zheng X; Cheng Q; Yao F; Wang X; Kong L; Cao B; Xu M;   Lin S; Chooi YH; You D*. Biosynthesis of the pyrrolidine protein synthesis inhibitor   anisomycin involves novel gene ensemble and cryptic biosynthetic steps, Proc   Natl Acad Sci U S A., 2017.4.18, 114(16): 4135~4140

10.    Kong L, Zhang W, Chooi YH, Wang L, Cao B, Deng Z,   Chu Y, You D*. A Multifunctional Monooxygenase XanO4 Catalyzes Xanthone   Formation in Xantholipin Biosynthesis via a Cryptic Demethoxylation. Cell   Chem Biol. 2016, 23(4):508-16.

11.    Zheng T, Jiang P, Cao B, Cheng Q, Kong L, Zheng X,   Hu Q, You D*. DndEi Exhibits Helicase Activity Essential for DNA   Phosphorothioate Modification and ATPase Activity Strongly Stimulated by DNA   Substrate with a GAAC/GTTC Motif. J Biol Chem. 2016, 291   (3), 1492-500.

12.    Cao B, Zheng X, Cheng Q, Yao F, Zheng T, Ramesh Babu I, Zhou H,   Dedon PC, You D*. In vitro analysis of phosphorothioate modification of DNA   reveals substrate recognition by a multiprotein complex. Sci Rep.   2015, 5, 12513.

13.    Cheng Q#, Cao B#, Yao F, Li J, Deng Z, You D*.   Regulation of DNA phosphorothioate modifications by the transcriptional   regulator DptB in Salmonella. Mol Microbiol. 2015, 97   (6), 1186-94.

14.    Cao B#, Chen C#, DeMott MS#, Cheng Q, Clark TA, Xiong X, Zheng X,   Butty V, Levine SS, Yuan G, Boitano M, Luong K, Song Y, Zhou X, Deng Z,   Turner SW, Korlach J, You D*, Wang L*, Chen S*, Dedon PC. Genomic mapping of   phosphorothioates reveals partial modification of short consensus sequences. Nat   Commun. 2014, 5, 3951.

15.    Cao B, Cheng Q, Gu C, Yao F, DeMott MS, Zheng X, Deng Z, Dedon   PC*, You D*. Pathological phenotypes and in vivo DNA cleavage by unrestrained   activity of a phosphorothioate-based restriction system in Salmonella. Mol   Microbiol. 2014, 93 (4), 776-85.

16.  Cao B, Yao   F, Zheng X, Cui D, Shao Y, Zhu C, Deng Z, You D*. Genome mining of the   biosynthetic gene cluster of the polyene macrolide antibiotic tetramycin and   characterization of a P450 monooxygenase involved in the hydroxylation of the   tetramycin B polyol segment. Chembiochem. 2012, 13 (15),   2234-42.

专利技术

1. 曹博,一种定位基因组DNA上损伤和修饰位点的方法,2019.8.9,中国专利,授权号:ZL   201811145591.6

2. 曹博,一种对RNA分子进行绝对定量的高通量测序方法,2019.7.9,中国专利,授权号:ZL   201811145601.6

3. 曹博,张清华,刘莉莉,季红,一种DNA 可逆保护和分离的方法,2020.6.11,中国专利,申请号: 202010528082.2

4. Bo Cao;   Peter C. Dedon; Jennifer F. Hu; Michael S. DeMott, METHODS OF QUANTIFYING RNA   AND DNA VARIANTS THROUGH SEQUENCING EMPLOYING PHOSPHOROTHIOATES, 2018.3.15, 美国专利,授权号:M0656.70437US00,国际专利,授权号:PCT/US2019/013714

5. Peter C. Dedon;   Delin You; Bo Cao; Ramesh Babu Indrakanti; Michael S. DeMott; Lianrong   Wang; Zixin Deng; Xiufen Zhou, Enzymatic phosphorothioation of DNA or RNA,   2014.10.16, 美国专利,授权号:   US20140308711A1, 国际专利,授权号:   PCT/US2014/026241

6.  由德林; 曹博; 邓子新; 郑晓青, 磷硫酰化DNA酶促合成方法, 2014.12.10, 中国专利,授权号: ZL 2012 1   0091733.1

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